A new restriction endonuclease LmnI recognizes the nonpalindromic DNA sequence 5’-GCTCC(1/-1)-3’
Valery A. Chernukhin, Danila A. Gonchar, Murat A. Abdurashitov, Tatyana N. Nayakshina, Vladimir S. Dedkov, Natalya A. Mikhnenkova, Elena N. Lomakovskaya and Sergey Kh. Degtyarev
We have discovered a bacterial strain Lysinibacillus manganicus An22 that produces the new prototype of restriction endonuclease named LmnI. This enzyme recognizes a nonpalindromic DNA sequence 5’-GCTCC-3’/3’-CGAGG-5’. The LmnI restriction endonuclease preparation with the concentration of 1000 units/ml was isolated using four chromatographic steps. It was shown that new enzyme cuts its recognition sequence forming 3’-protruding ends as indicated by the arrows: 5’-GCTCCN↓-3’/3’-CGAG↑GN-5’, and can be applied to a IIS type of restriction-modification systems.