{"id":436,"date":"2025-11-18T18:30:55","date_gmt":"2025-11-18T11:30:55","guid":{"rendered":"https:\/\/dnape.online\/?p=436"},"modified":"2025-11-19T10:48:17","modified_gmt":"2025-11-19T03:48:17","slug":"bmoi","status":"publish","type":"post","link":"https:\/\/dnape.online\/en\/2025\/11\/bmoi\/","title":{"rendered":"BmoI Restriction Endonuclease from the Bacterial Strain Brevundimonas mongoliensis 53 Recognizes the DNA Sequence 5\u2019-GG^GWCCC-3\u2019"},"content":{"rendered":"\n<\/span>

[vc_row][vc_column width=”2\/3″][vc_column_text]D.A. Gonchar1<\/sup>, M.A. Abdurashitov1<\/sup>, V.A. Chernukhin1<\/sup>, V.S. Dedkov1<\/sup>, N.A. Mikhnenkova1<\/sup>, V.E. Ryzhova1<\/sup>, S.Kh. Degtyaryov1<\/sup><\/strong><\/p>\n

1<\/sup> SibEnzyme Ltd, Novosibirsk, Russia<\/p>\n

* corresponding author: D.A. Gonchar, SibEnzyme Ltd, 2\/12 Timakova Str.,
\nNovosibirsk 630117, Russia;
\nphone: +7(383)333-4991; fax: +7(383)333-6853;
\nE-mail: gonchar@sibenzyme.ru<\/a><\/p>\n

We identified a bacterial strain, Brevundimonas mongoliensis<\/em> 53, which produces a new Type II restriction endonuclease, designated BmoI.
BmoI recognizes the palindromic heptanucleotide sequence 5\u2019-GG^GWCCC-3\u2019 (W = A or T) and cleaves at the indicated position to generate three-nucleotide 5\u2032 overhangs.
BmoI is an isoschizomer of the previously described restriction enzyme SanDI.
The producing strain was identified based on morphological and biochemical characteristics and by sequencing a fragment of the 16S rRNA gene.
A preparation of BmoI with an activity of 2000 U\/ml was obtained using a three-step chromatographic purification workflow.
Optimal reaction conditions for BmoI were determined to be SE buffer W (10 mM Tris-HCl, pH 8.5; 10 mM MgCl\u2082; 100 mM NaCl; 1 mM DTT) and an incubation temperature of 37\u00b0C.<\/p>\n

List of abbreviations:<\/em><\/strong>
\nBSA \u2013 bovine serum albumin;
\nDTT \u2013 dithiothreitol;
\nT7 DNA \u2013 bacteriophage T7 DNA;
\n\u03bb DNA \u2013 bacteriophage \u03bb DNA;
\nU \u2013 enzyme units;
\nTris \u2013 tris(hydroxymethyl)aminomethane;
\nPAGE \u2013 polyacrylamide gel;
\nbp \u2013 base pairs;
\nRE \u2013 restriction endonuclease;
\nEDTA \u2013 ethylenediaminetetraacetic acid.[\/vc_column_text][\/vc_column][vc_column width=”1\/3″][vc_column_text]Keywords:<\/strong> producer strain, enzyme purification, restriction endonuclease, isoschizomer<\/p>\n

DOI:<\/strong> 10.26213\/3034-4301.2025.5.2.002<\/strong><\/p>\n

Citation:<\/strong>
\nGonchar D.A., Abdurashitov M.A., Chernukhin V.A., Dedkov V.S., Mikhnenkova N.A., Ryzhova V.E., Degtyaryov S.Kh. (2025) BmoI restriction endonuclease from the bacterium Brevundimonas mongoliensis<\/em> 53 recognizes the DNA sequence 5\u2019-GG^GWCCC-3\u2019. \/\/ DNA-Processing Enzymes<\/em>, 2025(2), DOI: 10.26213\/3034-4301.2025.5.2.002<\/p>\n

\"Creative<\/a>
\nThis article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License<\/a>[\/vc_column_text][\/vc_column][\/vc_row]<\/p>","protected":false},"excerpt":{"rendered":"

We identified a bacterial strain, Brevundimonas mongoliensis 53, which produces a new Type II restriction endonuclease, designated BmoI.
\nBmoI recognizes the palindromic heptanucleotide sequence 5\u2019-GG^GWCCC-3\u2019 (W = A or T) and cleaves at the indicated position to generate three-nucleotide 5\u2032 overhangs.
\nBmoI is an isoschizomer of the previously described restriction enzyme SanDI.
\nThe producing strain was identified based on morphological and biochemical characteristics and by sequencing a fragment of the 16S rRNA gene.
\nA preparation of BmoI with an activity of 2000 U\/ml was obtained using a three-step chromatographic purification workflow.
\nOptimal reaction conditions for BmoI were determined to be SE buffer W (10 mM Tris-HCl, pH 8.5; 10 mM MgCl\u2082; 100 mM NaCl; 1 mM DTT) and an incubation temperature of 37\u00b0C.<\/p>\n","protected":false},"author":1,"featured_media":466,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[5,45],"tags":[47],"coauthors":[9,10,8,12,13,46,16],"class_list":["post-436","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-new-enzymes","category-45","tag-bmoi"],"_links":{"self":[{"href":"https:\/\/dnape.online\/en\/wp-json\/wp\/v2\/posts\/436","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/dnape.online\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/dnape.online\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/dnape.online\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/dnape.online\/en\/wp-json\/wp\/v2\/comments?post=436"}],"version-history":[{"count":17,"href":"https:\/\/dnape.online\/en\/wp-json\/wp\/v2\/posts\/436\/revisions"}],"predecessor-version":[{"id":461,"href":"https:\/\/dnape.online\/en\/wp-json\/wp\/v2\/posts\/436\/revisions\/461"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/dnape.online\/en\/wp-json\/wp\/v2\/media\/466"}],"wp:attachment":[{"href":"https:\/\/dnape.online\/en\/wp-json\/wp\/v2\/media?parent=436"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/dnape.online\/en\/wp-json\/wp\/v2\/categories?post=436"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/dnape.online\/en\/wp-json\/wp\/v2\/tags?post=436"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/dnape.online\/en\/wp-json\/wp\/v2\/coauthors?post=436"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}