{"id":369,"date":"2024-10-28T16:50:17","date_gmt":"2024-10-28T09:50:17","guid":{"rendered":"https:\/\/dnape.online\/?p=369"},"modified":"2025-12-26T11:48:46","modified_gmt":"2025-12-26T04:48:46","slug":"peci","status":"publish","type":"post","link":"https:\/\/dnape.online\/en\/2024\/10\/peci\/","title":{"rendered":"The restriction endonuclease PecI from the bacterial strain Paracoccus species 12 recognizes the DNA sequence 5\u2019-TTA^TAA-3\u2019 and is an isoschizomer of PsiI"},"content":{"rendered":"\n<\/span>

[vc_row css=”.vc_custom_1576496196814{background-color: #ededed !important;}”][vc_column width=”2\/3″][vc_column_text]D.A. Gonchar1<\/sup>, V.A. Chernukhin1<\/sup>, V.S. Dedkov1<\/sup>, N.A. Mikhnenkova1<\/sup>, M.A. Abdurashitov1<\/sup>, O.A. Belichenko1<\/sup>, S.Kh. Degtyarev1<\/sup><\/p>\n

1<\/sup> SibEnzyme Ltd, Novosibirsk<\/p>\n

Corresponding author: Danila Gonchar, SibEnzyme Ltd, 2\/12 Timakova St., Novosibirsk, 630117, Russia; Tel.: +7(383)333-4991; Fax: +7(383)333-6853; E-mail: gonchar@sibenzyme.ru<\/a><\/p>\n

We identified a bacterial strain, Paracoccus species 12<\/em>, which produces a novel restriction endonuclease named PecI. This enzyme recognizes the hexanucleotide palindromic DNA sequence 5\u2019-TTA^TAA-3\u2019 and cleaves it, as indicated by the arrow, generating blunt ends. Thus, PecI is a true isoschizomer of the restriction enzyme PsiI [1].<\/p>\n

A preparation of PecI restriction endonuclease with a concentration of 10,000 U\/mL was obtained through a four-step chromatographic purification process. The optimal reaction conditions for the enzyme are SE-buffer 5 (Y) (33 mM Tris-acetate (pH 7.9), 10 mM Mg-acetate, 66 mM K-acetate, 1 mM DTT) and a temperature of 37\u00b0C.<\/p>\n

Used abbreviations:<\/strong>
\nBSA \u2013 bovine serum albumin; DTT \u2013 dithiothreitol; PAGE \u2013 polyacrylamide gel electrophoresis; bp \u2013 base pairs; RM system \u2013 restriction-modification system; Tris \u2013 tris(hydroxymethyl)aminomethane; EDTA \u2013 ethylenediaminetetraacetic acid; RE \u2013 restriction endonuclease.[\/vc_column_text][\/vc_column][vc_column width=”1\/3″][vc_column_text]Keywords:<\/strong> restriction endonucleases, isoschizomers<\/p>\n

DOI:<\/strong><\/p>\n

Citation details:<\/strong>
\nD.A. Gonchar, V.A. Chernukhin, V.S. Dedkov, N.A. Mikhnenkova, M.A. Abdurashitov, O.A. Belichenko, S.Kh. Degtyarev. (2024) The restriction endonuclease PecI from the bacterial strain Paracoccus species 12<\/em> recognizes the DNA sequence 5\u2019-TTA^TAA-3\u2019 and is an isoschizomer of PsiI, DNA processing enzymes, Volume 2024(1), DOI:<\/p>\n

\"\u041b\u0438\u0446\u0435\u043d\u0437\u0438\u044f<\/a>
\nThis article is available under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International<\/a><\/p>\n

Full-text pdf[\/vc_column_text][\/vc_column][\/vc_row]<\/p>","protected":false},"excerpt":{"rendered":"

We identified a bacterial strain, Paracoccus species 12<\/em>, which produces a novel restriction endonuclease, named PecI. This enzyme recognizes the hexanucleotide palindromic DNA sequence 5\u2019-TTA^TAA-3\u2019 and cleaves it, as indicated by the arrow, generating blunt ends. Thus, PecI is a true isoschizomer of the restriction enzyme PsiI.<\/p>\n

A preparation of PecI restriction endonuclease at a concentration of 10,000 U\/mL was obtained through a four-step chromatographic purification process. The optimal reaction conditions for the enzyme are SE-buffer 5 (Y) (33 mM Tris-acetate (pH 7.9), 10 mM Mg-acetate, 66 mM K-acetate, 1 mM DTT) and a temperature of 37o<\/sup>C.<\/p>\n","protected":false},"author":4,"featured_media":428,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[5,45],"tags":[],"coauthors":[9,8,12,13,10,16],"class_list":["post-369","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-new-enzymes","category-45"],"_links":{"self":[{"href":"https:\/\/dnape.online\/en\/wp-json\/wp\/v2\/posts\/369","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/dnape.online\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/dnape.online\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/dnape.online\/en\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/dnape.online\/en\/wp-json\/wp\/v2\/comments?post=369"}],"version-history":[{"count":11,"href":"https:\/\/dnape.online\/en\/wp-json\/wp\/v2\/posts\/369\/revisions"}],"predecessor-version":[{"id":395,"href":"https:\/\/dnape.online\/en\/wp-json\/wp\/v2\/posts\/369\/revisions\/395"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/dnape.online\/en\/wp-json\/wp\/v2\/media\/428"}],"wp:attachment":[{"href":"https:\/\/dnape.online\/en\/wp-json\/wp\/v2\/media?parent=369"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/dnape.online\/en\/wp-json\/wp\/v2\/categories?post=369"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/dnape.online\/en\/wp-json\/wp\/v2\/tags?post=369"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/dnape.online\/en\/wp-json\/wp\/v2\/coauthors?post=369"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}